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KMID : 0380020030180010074
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 1 p.74 ~ p.77
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Rapid Analysis of Major Putrefactive metabolites by GC and GC/MSD
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Park Gyu-Yong
Kim Min-Cheol
Woo Kang-Lyung
Lee Na-Kyoung
Paik Hyun-Dong
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Abstract
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A simple, reproducible, and rapid gas chromatographic method for putrefactive metabolite determination in feces was developed. The method involves the direct injection of fecal supernatants into the gas chromatograph, without pretreatment. The mass spectra of these metabolites were obtained using an HP 5971 mass selective detector operated in electron impact (EI) ionization mode. This method produced sharp peaks and allowed the simultaneous determination of fecal putrefactive metabolites.
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KEYWORD
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Gas chromatography, putrefactive metabolite, feces, indole, skatole, ρ-cresol
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